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Cell Biology
Inhibitors
Phosphatase Inhibitor Cocktail (100X)
Phosphatase Inhibitor Cocktail (100X) from Cell Signaling Technology
Be the first to write a review!
Citations:
(96)
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Cell Signaling Technology for
Phosphatase Inhibitor Cocktail (100X)
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Reviews
Product Specs
Item
Phosphatase Inhibitor Cocktail (100X)
Company
Cell Signaling Technology
Price
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Catalog Number
5870S
Quantity
1 ml
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Cell Signaling Technology
3 Trask Lane
Danvers
,
Massachusetts
01923
United States
Phone
: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488
Company Profile
Website:
http://www.cellsignal.com/
Website:
Technical Support
Citations (96)
(1)
AMP-activated protein kinase is necessary for Treg cell functional adaptation to microenvironmental stress
bioRxiv
December 1, 2023
Manuel A. Torres Acosta, Nurbek Mambetsariev, Carla P Reyes Reyes Flores, Kathryn A. Helmin, Qianli Liu, Anthony M. Joudi, Luisa Morales-Nebreda, Jonathan Gurkan, Kathleen Cheng, Hiam Abdala-Valencia,
et al.
lysis buffer (
Cell Signaling
cat. no.
9803
) supplemented with phosphatase (
Cell Signaling
cat. no.
5870S
) and
protease inhibitors
(Roche, cat. no. 65726900) after which their concentration was measured with
(2)
Scaffold-induced compression enhances ligamentization potential of decellularized tendon graft reseeded with ACL-derived cells
iScience
November 23, 2023
Jinsung Park, Hyunsoo Soh, Sungsin Jo, Subin Weon, Seung Hoon Lee, Jeong-Ah Park, Myung-Kyu Lee, Tae-Hwan Kim, Il-Hoon Sung, Jin Kyu Lee
following the conventional methods with 1x RIPA buffer containing phosphatase
inhibitor
(
Cell Signaling
,
5870S
) and protease
inhibitor
(Calbiochem, 535140) on ACL-derived cells. Quantification of extracted proteins
(3)
Adrenomedullin has a pivotal role in trophoblast differentiation: A promising nanotechnology-based therapeutic target for early-onset preeclampsia
Science Advances
November 3, 2023
Qingqing Zhang, Cheuk-Lun Lee, Tingyu Yang, Jianlin Li, Qunxiong Zeng, Xiaofeng Liu, Zhongzhen Liu, Degong Ruan, Zhuoxuan Li, Anita S. Y. Kan,
et al.
containing 1% proteinase
inhibitor
cocktail (11836170001, Roche) and 1% phosphatase
inhibitor
cocktail (
5870
,
Cell Signaling Technology
) followed by
centrifugation
at 12,000 rpm and 4°C for 30 min. Protein concentrations
(4)
Elevated BMPR2 expression amplifies osteoblast differentiation in ankylosing spondylitis
Journal of Rheumatic Diseases
October 1, 2023
Sungsin Jo, Seung Hoon Lee, Chanhyeok Jeon, Hye-Ryeong Jo, Eunae Ko, Min Whangbo, Tae-Jong Kim, Ye-Soo Park, Tae-Hwan Kim
Thermo Fisher Scientific
). The cells were lysed with 1×
radioimmunoprecipitation assay
buffer containing phosphatase (
5870S
;
Cell Signaling Technology
, Danvers, MA, USA) and protease (#535140; Calbiochem, San Diego, CA, USA)
(5)
Selective Activation of M1 Muscarinic Receptors Attenuates Human Colon Cancer Cell Proliferation
Cancers (Basel)
September 28, 2023
Margaret H. Sundel, Natalia Sampaio Moura, Kunrong Cheng, Oscar Chatain, Shien Hu, Cinthia B. Drachenberg, Guofeng Xie, Jean-Pierre Raufman
containing Cell Lysis Buffer (catalog #
9803
), anti-protease (catalog #
5871
), and anti-phosphatase (catalog #
5870
)—all from
Cell Signaling
. Cell lysates were centrifuged at 15,000× g at 4 °C for 10 min. Supernatants
(6)
HER2-Selective and Reversible Tyrosine Kinase Inhibitor Tucatinib Potentiates the Activity of T-DM1 in Preclinical Models of HER2-positive Breast Cancer
Cancer Research Communications
September 25, 2023
Devra Olson, Janelle Taylor, Kelsi Willis, Kelly Hensley, Sean Allred, Margo Zaval, Lauren Farr, Robert Thurman, Nishi Jain, Renee Hein,
et al.
Protease Inhibitor Cocktail (Millipore Sigma; #535140) and
1:100
Phosphatase Inhibitor Cocktail (
CST
; #
5870S
). Protein concentration of lysates was measured using a bicinchoninic acid
assay
. Protein levels were
(7)
NRP1 transduces mechanical stress inhibition via LATS1/YAP in hypertrophic scars
Cell Death Discovery
September 13, 2023
Mengzhi Li, Peng Wang, Jingting Li, Fei Zhou, Shixin Huang, Shaohai Qi, Bin Shu
USA) containing a protease
inhibitor
cocktail and phosphatase
inhibitor
cocktail (
1:100
; #
5871
and #
5870
, respectively;
Cell Signaling Technology
, Danvers, MA, USA). Briefly, 20 µl of each sample was loaded
(8)
Δ9-Tetrahydrocannabinol does not upregulate an aversive dopamine receptor mechanism in adolescent brain unlike in adults
Current Research in Neurobiology
September 4, 2023
Marie-Eve Di Raddo, Marija Milenkovic, Meenalochani Sivasubramanian, Ahmed Hasbi, Jack Bergman, Sarah Withey, Bertha K. Madras, Susan R. George
containing protease
inhibitor
(Sigma, P8340) and phosphatase
inhibitor
(
Cell Signaling Technology
,
5870
S). Samples were maintained in constant agitation for 30 min then centrifuged at 10 000 g for 10 min
(9)
Inhibition of DNMT1 methyltransferase activity via glucose-regulated O-GlcNAcylation alters the epigenome
eLife
July 20, 2023
Shin H, Leung A, Costello KR, Senapati P, Kato H, Moore RE, Lee M, Lin D, Tang X, Pirrotte P,
et al
.
presence of a protease
inhibitor
(Cat# 8340; Sigma-Aldrich) and a phosphatase
inhibitor
cocktail (Cat#
5870
;
Cell Signaling
) for the
western blotting
or
immunoprecipitation
. An increase in fasting blood glucose
(10)
Mechanism and therapeutic implications of pomalidomide-induced immune surface marker upregulation in EBV-positive lymphomas
Scientific reports
July 18, 2023
Jaeger HK, Davis DA, Nair A, Shrestha P, Stream A, Yaparla A, Yarchoan R.
phosphorylated
AKT
were extracted in the presence of both phosphatase
inhibitor
cocktail (
Cell signaling
cat#
5870
) and protease
inhibitor
cocktail (ThermoFisher Scientific) at a final 1X concentration. Protein concentrations
(11)
SMYD5 is a novel epigenetic gatekeeper of the mild hypothermia response
bioRxiv : the preprint server for biology
June 9, 2023
Rafnsdottir S, Jang K, Halldorsdottir ST, Tomasdottir A, Vinod M, Möller K, Reynisdottir T, Atladottir LH, Allison KE, He J,
et al
.
1% NP-40, 1% Sodium deoxycholate, 0.1% SDS, 2mM EDTA, phosphatase
inhibitor
(either
Cell signaling
,
5870S
or
Thermo Fisher Scientific
,
78437
)). The lysate was centrifuged at 16,000 ×g for 20 min at 4°C and
(12)
Hyponatremia-related liver steatofibrosis and impaired spermatogenesis: evidence from a mouse model of the syndrome of inappropriate antidiuresis
Journal of endocrinological investigation
May 1, 2023
Marroncini G, Anceschi C, Naldi L, Fibbi B, Brogi M, Lanzilao L, Fanelli A, Maggi M, Peri A.
supplemented with protease (11,697,498,001, Roche, Basel, Switzerland) and phosphatase
inhibitor
cocktail (#
5870
,
Cell Signaling Technology
, Danvers, Massachusetts, USA) 100X, to obtain whole protein lysate for
immunoblot
(13)
Phosphatase regulatory subunit MYPT2 knockout partially compensates for the cardiac dysfunction in mice caused by lack of myosin light chain kinase 3
Journal of Biological Chemistry
April 1, 2023
Tingfei Hu, Hema Kalyanaraman, Renate B. Pilz, Darren E. Casteel
Corporation (Cat#: 539131) and phosphatase
inhibitor
cocktail was from
Cell Signaling Technology
(Cat#:
5870
). Horseradish peroxidase (HRP)-conjugated anti-Flag antibody was from
Sigma-Aldrich
(Cat#:
A8592
). Rabbit
(14)
Activation of the PI3K/AKT/mTOR Pathway in Cajal–Retzius Cells Leads to Their Survival and Increases Susceptibility to Kainate-Induced Seizures
International Journal of Molecular Sciences
March 11, 2023
Nasim Ramezanidoraki, Driss El Ouardi, Margaux Le, Stéphanie Moriceau, Mahboubeh Ahmadi, Elena Dossi, Danae Rolland, Philippe Bun, Gwenaëlle Le Pen, Guillaume Canaud,
et al.
(Saint-Louis, MI, USA) with protease (ref: #04693116001; Roche (Bâle, Switzerland) and phosphatase (ref: #
5870
;
Cell Signaling
(Danvers, MA, USA))
inhibitors
. Following this, 25 µg of the total protein extracts
(15)
Extracellular PPM1A promotes mineralization of osteoblasts differentiation in ankylosing spondylitis via the FOXO1A‐RUNX2 pathway
Journal of Cellular and Molecular Medicine
March 1, 2023
Subin Weon, Sungsin Jo, Bora Nam, Sung Hoon Choi, Ye‐Soo Park, Yong‐Gil Kim, Tae‐Hwan Kim
X‐100) containing
protease inhibitors
(Calbiochem, 535140) and phosphatase
inhibitors
(
Cell Signalling
,
5870
S). Lysed samples were incubated on ice for 15 min followed by
centrifugation
at 12,000× g for 15 min
(16)
New Glucosamine-Based TLR4 Agonists: Design, Synthesis, Mechanism of Action, and In Vivo Activity as Vaccine Adjuvants
Journal of Medicinal Chemistry
February 23, 2023
Alessio Romerio, Nicole Gotri, Ana Rita Franco, Valentina Artusa, Mohammed Monsoor Shaik, Samuel T. Pasco, Unai Atxabal, Alejandra Matamoros-Recio, Marina Mínguez-Toral, Juan Diego Zalamea,
et al.
(
CST
, #
9806
), supplemented with protease (Roche, Mannheim, Germany) and phosphatase
inhibitors
(
CST
##
5870
). After
centrifugation
at 13,000 RCF for 30 min at 4 °C, the supernatants were collected as whole-cell
(17)
TRPC7 facilitates cell growth and migration by regulating intracellular Ca2+ mobilization in lung adenocarcinoma cells
Oncology Letters
January 24, 2023
Jui-Lin Liang, Ming-Hsien Tsai, Yi-Chun Hsieh, Huei-Syuan Liu, Shao-Wei Chen, Yung-Yun Huang, Li-Ching Lin, Tsung-Fu Tsai, Yun-Fang Liang, Wen-Li Hsu
containing proteinase (cat. no. S8830-20TAB; Sigma-Aldrich; Merck KGaA) and phosphatase
inhibitors
(cat. no.
5870S
;
Cell Signaling Technology
, Inc.). Cell debris were removed by
centrifugation
at 14,000 × g for 30 min
(18)
Torin1 restores proliferation rate in Charcot-Marie-Tooth disease type 2A cells harbouring MFN2 (mitofusin 2) mutation
Acta Myol
December 31, 2022
Paola Zanfardino, Alessandro Amati, Easter Anna Petracca, Filippo M. Santorelli, Vittoria Petruzzella
Scientific, 89900) supplemented with proteases (
Cell Signaling
,
5871
) and phosphatase (
Cell Signaling
,
5870S
)
inhibitors
. The cells were sonicated on ice and centrifuged for 10 min at 16,000×g at 4°C and the protein
(19)
1α, 25-Dihydroxyvitamin D3 protects gastric mucosa epithelial cells against
Helicobacter pylori
-infected apoptosis through a vitamin D receptor-dependent c-Raf/MEK/ERK pathway
Pharmaceutical Biology
December 31, 2022
Shuai Zhao, Daihong Wan, Yaoyao Zhong, Xiwei Xu
USA). RIPA buffer (#
9806
), protease
inhibitor
cocktail (#
7012
) and phosphatase
inhibitor
cocktail (#
5870
) were purchased from
Cell Signalling
Technology. Cell and bacterial culture
Human
gastric mucosa
epithelial cell
(20)
CYFIP1 Dosages Exhibit Divergent Behavioral Impact via Diametric Regulation of NMDA Receptor Complex Translation in Mouse Models of Psychiatric Disorders
Biological Psychiatry
November 1, 2022
Nam-Shik Kim, Francisca Rojas Ringeling, Ying Zhou, Ha Nam Nguyen, Stephanie J. Temme, Yu-Ting Lin, Stephen Eacker, Valina L. Dawson, Ted M. Dawson, Bo Xiao,
et al.
cocktail,Roche,11873580001, Chemical Compound or Drug,Phosphatase Inhibitor Cocktail,
Cell Signaling
,
5870
, Chemical Compound or Drug,Protease Inhibitor Cocktail,Sigma,P8340, Chemical Compound or Drug,4x
Laemmli Sample Buffer
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Phosphatase Inhibitor Cocktail (100X) from Cell Signaling Technology
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
Citations
Reviews
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